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Gli1 antibody

Anti-Gli1 antibody used in Western Blot (WB). GTX17495

Cat. No. GTX17495

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Gli1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of Gli1. The exact sequence is proprietary.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887565

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GLI family zinc finger 1 , GLI , PAPA8

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-Gli1 antibody used in Western Blot (WB). GTX17495

GTX17495 WB Image

WB analysis of PC3 (A), mouse brain (B), rat brain (C) whole cell lysates using GTX17495 Gli1 antibody.

REFERENCE

Application Reference

Yang JS et al. Oncol Rep 2018; 39 (1) : 383-391 ITR-284 modulates cell differentiation in human chronic myelogenous leukemia K562 cells.
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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399