HIF1 beta antibody [2B10]

Cat. No. GTX22771

Reference 7

Host	Mouse
Clonality	Monoclonal
Clone Name	2B10
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IP, Gel supershift assays, IHC
Reactivity	Human, Mouse, Rat, Xenopus, Fish, Primate
Package	100 μl ($399)

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View product citations for antibody GTX22771 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:1,000
IHC-P	5 μg/ml
IP	Assay dependent
Gel supershift assays	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues C N(771) S Y N N E E F P D L T M F P P F S E(789) of human ARNT.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aryl hydrocarbon receptor nuclear translocator , HIF-1-beta , HIF-1beta , HIF1-beta , HIF1B , HIF1BETA , TANGO , bHLHe2

Cellular Localization

Nucleus

Background

This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]

Database

Gene ID: 405 ARNT
UniProt: P27540 ARNT

Research Area

- Cancer
- Cancer
- Hypoxia

DATA IMAGES

GTX22771 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22771 HIF1 beta antibody [2B10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

GTX22771 ICC/IF Image

ICC/IF analysis of NRK cells using GTX22771 HIF1 beta antibody [2B10].
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes
Dilution : 40 μg/ml in staining buffer for 1 hour at room temperature

GTX22771 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22771 HIF1 beta antibody [2B10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

GTX22771 WB Image

WB analysis of whole cell extracts (30 μg lysate) of HeLa (Lane 1), MCF-7 (Lane 2), NIH/3T3 (lane 3) and PC 12 (Lane 4) using GTX22771 HIF1 beta antibody [2B10].
Dilution : 1:500-3000

GTX22771 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX22771 HIF1 beta antibody [2B10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC