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IGF2BP2 antibody

Anti-IGF2BP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113922
Anti-IGF2BP2 antibody used in Western Blot (WB). GTX113922
Anti-IGF2BP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113922

Cat. No. GTX113922

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IGF2BP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IGF2BP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037231

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor 2 mRNA binding protein 2 , IMP-2 , IMP2 , VICKZ2

Cellular Localization

Cytoplasm (Probable)

Background

This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IGF2BP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113922

GTX113922 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IGF2BP2(GTX113922) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IGF2BP2 antibody used in Western Blot (WB). GTX113922

GTX113922 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF2BP2 antibody (GTX113922) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IGF2BP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113922

GTX113922 ICC/IF Image

IGF2BP2 antibody [N1N3] detects IGF2BP2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IGF2BP2 protein stained by IGF2BP2 antibody [N1N3] (GTX113922) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

Application Reference

Mohamed Elbadawy et al. Biomaterials 2020; (Epub) Efficacy of primary liver organoid culture from different stages of non-alcoholic steatohepatitis (NASH) mouse model.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169