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IKK gamma (phospho Ser85) antibody

Anti-IKK gamma (phospho Ser85) antibody used in Western Blot (WB). GTX32283
Anti-IKK gamma (phospho Ser85) antibody used in IHC (Paraffin sections) (IHC-P). GTX32283

Cat. No. GTX32283

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of IKK gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887648

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of nuclear factor kappa B kinase regulatory subunit gamma , AMCBX1 , EDAID1 , FIP-3 , FIP3 , Fip3p , IKK-gamma , IKKAP1 , IKKG , IMD33 , IP , IP1 , IP2 , IPD2 , NEMO , ZC2HC9

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-IKK gamma (phospho Ser85) antibody used in Western Blot (WB). GTX32283

GTX32283 WB Image

WB analysis of LPS-treated THP1 (A), LPS-treated Raw264.7 (B), rat brain (C) whole cell lysates using GTX32283 IKK gamma (phospho Ser85) antibody.

Anti-IKK gamma (phospho Ser85) antibody used in IHC (Paraffin sections) (IHC-P). GTX32283

GTX32283 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32283 IKK gamma (phospho Ser85) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399