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ILK antibody [3A9]

Anti-ILK antibody [3A9] used in Flow cytometry (FACS). GTX60645
Anti-ILK antibody [3A9] used in IHC (Paraffin sections) (IHC-P). GTX60645
Anti-ILK antibody [3A9] used in Western Blot (WB). GTX60645
Anti-ILK antibody [3A9] used in IHC (Paraffin sections) (IHC-P). GTX60645
Anti-ILK antibody [3A9] used in ELISA (ELISA). GTX60645

Cat. No. GTX60645

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3A9

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($409)
See all ILK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ILK (AA: 97-244) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin linked kinase , HEL-S-28 , ILK-1 , ILK-2 , P59 , p59ILK

Background

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Database

Research Area

DATA IMAGES

Anti-ILK antibody [3A9] used in Flow cytometry (FACS). GTX60645

GTX60645 FACS Image

FACS analysis of Jurkat cells using GTX60645 ILK antibody [3A9].
Green : ILK
Red : negative control

Anti-ILK antibody [3A9] used in IHC (Paraffin sections) (IHC-P). GTX60645

GTX60645 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60645 ILK antibody [3A9].

Anti-ILK antibody [3A9] used in Western Blot (WB). GTX60645

GTX60645 WB Image

WB analysis of Jurkat (1), NIH3T3 (2), HeLa (3), PC-12 (4), C6 (5), COS7 (6), Raji (7), K562 (8) and MCF-7 (9) cell lysate using GTX60645 ILK antibody [3A9].

Anti-ILK antibody [3A9] used in IHC (Paraffin sections) (IHC-P). GTX60645

GTX60645 IHC-P Image

IHC-P analysis of esophageal cancer tissue using GTX60645 ILK antibody [3A9].

Anti-ILK antibody [3A9] used in ELISA (ELISA). GTX60645

GTX60645 ELISA Image

ELISA analysis of antigen using GTX60645 ILK antibody [3A9].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409