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Integrin alpha 4 antibody [5C4]

Anti-Integrin alpha 4 antibody [5C4] used in ELISA (ELISA). GTX60576
Anti-Integrin alpha 4 antibody [5C4] used in Western Blot (WB). GTX60576
Anti-Integrin alpha 4 antibody [5C4] used in Western Blot (WB). GTX60576
Anti-Integrin alpha 4 antibody [5C4] used in Flow cytometry (FACS). GTX60576

Cat. No. GTX60576

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5C4

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all Integrin alpha 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ITGA4 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha 4 , CD49D , IA4

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Integrin alpha 4 antibody [5C4] used in ELISA (ELISA). GTX60576

GTX60576 ELISA Image

ELISA analysis of antigen using GTX60576 Integrin alpha 4 antibody [5C4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Integrin alpha 4 antibody [5C4] used in Western Blot (WB). GTX60576

GTX60576 WB Image

WB analysis of K562(1) and Jurkat (2) cell lysate using GTX60576 Integrin alpha 4 antibody [5C4].

Anti-Integrin alpha 4 antibody [5C4] used in Western Blot (WB). GTX60576

GTX60576 WB Image

WB analysis of human ITGA4 (AA: 926-978) recombinant protein using GTX60576 Integrin alpha 4 antibody [5C4].

Anti-Integrin alpha 4 antibody [5C4] used in Flow cytometry (FACS). GTX60576

GTX60576 FACS Image

FACS analysis of Jurkat cells using GTX60576 Integrin alpha 4 antibody [5C4].
Green : Integrin alpha 4
Red : negative control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409