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JNK1 + JNK2 antibody

Overexpression
Anti-JNK1 + JNK2 antibody used in Western Blot (WB). GTX133806
Anti-JNK1 + JNK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133806
Anti-JNK1 + JNK2 antibody used in Western Blot (WB). GTX133806

Cat. No. GTX133806

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all JNK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa , SK-N-AS , rat brain , JNK1 and JNK2-transfected 293T

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human JNK1 + JNK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887115

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

JNK1 antibody , JNK1A2 antibody , JNK21B1/2 antibody , JNK46 antibody , MAP kinase 8 antibody , MAPK 8 antibody , MAPK8 antibody , PRKM8 antibody , SAPK1 antibody , SAPK1c antibody , mitogen-activated protein kinase 8 antibody

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-JNK1 + JNK2 antibody used in Western Blot (WB). GTX133806

GTX133806 WB Image

Various whole cell and tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 + JNK2 antibody (GTX133806) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-JNK1 + JNK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133806

GTX133806 IHC-P Image

JNK1 + JNK2 antibody detects JNK1 + JNK2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
JNK1 + JNK2 stained by JNK1 + JNK2 antibody (GTX133806) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-JNK1 + JNK2 antibody used in Western Blot (WB). GTX133806

GTX133806 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 + JNK2 antibody (GTX133806) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Luo H et al. JCI Insight 2023; 8 (23) Signaling metabolite succinylacetone activates HIF-1α and promotes angiogenesis in GSTZ1-deficient hepatocellular carcinoma.
Yi Liu et al. Genes Dis 2021; 8 (2) : 232-240 Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway.
Application : WB
Reactivity : Human
Jau‐Tien Lin et al. Journal of Food Biochemistry 2019; (Epub) Litchi (Litchi chinensis Sonn.) flower proanthocyanidin fraction exhibited protective efficacy to suppress nickel‐induced expression for vascular endothelial growth factor in HepG2 cells.
Application : WB
Reactivity : Human
Huang C et al. Mol Ther Nucleic Acids 2019; 16 : 51-62 MEG3, as a Competing Endogenous RNA, Binds with miR-27a to Promote PHLPP2 Protein Translation and Impairs Bladder Cancer Invasion.
Application : WB
Reactivity : Human
Lin JT et al. Journal of Functional Foods 2018; 47 : 343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169