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Ki67 antibody [MSVA-267M] HistoMAX™

HistoMAX
Anti-Ki67 antibody [MSVA-267M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04361
Anti-Ki67 antibody [MSVA-267M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04361
Anti-Ki67 antibody [MSVA-267M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04361

Cat. No. GTX04361

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MSVA-267M

Isotype

 IgG2b

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($679),
100 μl ($299)
See all Ki67 products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 9.0 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
Not tested in other applications.

Calculated MW

359 kDa. ( Note )

Positive Control

Tonsil (moderate to strong nuclear staining in 80-90% of the germinal centre B-cells and in the vast majority of the suprabasal squamous epithelial cells).

Negative Control

Liver (nuclear staining reaction only in scattered hepatocytes (<1%), cytoplasmic staining is absent or only weak)

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Ki67 protein fragment (around aa 2293-2478)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

marker of proliferation Ki-67 , KIA , MIB- , MIB-1 , PPP1R105

Cellular Localization

Nucleus , Nucleolus

Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-Ki67 antibody [MSVA-267M] HistoMAX&trade; used in IHC (Paraffin sections) (IHC-P). GTX04361

GTX04361 IHC-P Image

IHC-P analysis of human breast cancer tissue section using GTX04361 Ki67 antibody [MSVA-267M] HistoMAX™
High Ki67 LI in a breast cancer of no special type (NST).

Anti-Ki67 antibody [MSVA-267M] HistoMAX&trade; used in IHC (Paraffin sections) (IHC-P). GTX04361

GTX04361 IHC-P Image

IHC-P analysis of human liver tissue section using GTX04361 Ki67 antibody [MSVA-267M] HistoMAX™
The liver represents an ideal normal tissue control for Ki67 staining quality. Less than 1 of the hepatocytes should stain and cytoplasmatic staining should be absent.

Anti-Ki67 antibody [MSVA-267M] HistoMAX&trade; used in IHC (Paraffin sections) (IHC-P). GTX04361

GTX04361 IHC-P Image

IHC-P analysis of human tonsil surface epithelium tissue section using GTX04361 Ki67 antibody [MSVA-267M] HistoMAX™

REFERENCE

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REVIEW

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Package List Price ($)
$ 679
$ 299