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LIMK1 antibody

Anti-LIMK1 antibody used in Western Blot (WB). GTX10561
Anti-LIMK1 antibody used in Western Blot (WB). GTX10561

Cat. No. GTX10561

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Mouse
Package
50 μg ($399)
See all LIMK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF Assay dependent
ELISA 1:8000-1:36000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to the C-terminal region near aa 620-647 of human LIM kinase protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

RRID

AB_422218

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LIM domain kinase 1 , LIMK , LIMK-1

Cellular Localization

Cytoplasm,Nucleus

Background

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase 1 and LIM kinase 2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Two splice variant have been identified.

Database

Research Area

DATA IMAGES

Anti-LIMK1 antibody used in Western Blot (WB). GTX10561

GTX10561 WB Image

Western blot using GeneTex Affinity Purified anti-LIM Kinase antibody (GTX10561) shows detection of a 73 kDa band corresponding to LIM kinase in lysates from mouse brain. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LIM kinase antibody. The doublet band at ~75 kDa may represent phosphorylated and non-phosphorylated forms of the protein. The identity of the strong lower molecular weight band at approximately 20 kDa is unknown. Signal was detected using standard techniques.

Anti-LIMK1 antibody used in Western Blot (WB). GTX10561

GTX10561 WB Image

WB analysis of mouse brain tissue lysate using GTX10561 LIMK1 antibody.
Loading : 18 μg
Dilution : 1:500

REFERENCE

Application Reference

Yu Q et al. Br J Pharmacol 2018; 175 (11) : 2077-2096 Inhibition of human prostate smooth muscle contraction by the LIM kinase inhibitors, SR7826 and LIMKi3.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399