MYH10 antibody [GT246]

Name: MYH10 antibody [GT246]
Brand: GeneTex
SKU: GTX634160
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

Anti-MYH10 antibody [GT246] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634160

Anti-MYH10 antibody [GT246] used in IHC (Paraffin sections) (IHC-P). GTX634160

Anti-MYH10 antibody [GT246] used in IHC (Frozen sections) (IHC-Fr). GTX634160

Cat. No. GTX634160

Reference 4

Host	Mouse
Clonality	Monoclonal
Clone Name	GT246
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, IHC-Fr
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all MYH10 products

View product citations for antibody GTX634160 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

229 kDa. ( Note )

Positive Control

HeLa , MYH10-transfected 293T , NIH-3T3 , mouse brain

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MYH10. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888411

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin heavy chain 10 , NMMHC-IIB , NMMHCB

Background

This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Database

Gene ID: 4628 MYH10
UniProt: P35580 MYH10

Research Area

DATA IMAGES

GTX634160 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX634160 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:5000.

GTX634160 ICC/IF Image

MYH10 antibody [GT246] detects MYH10 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MYH10 protein stained by MYH10 antibody [GT246] (GTX634160) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX634160 IHC-P Image

MYH10 antibody [GT246] detects MYH10 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MYH10 antibody [GT246] (GTX634160) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX634160 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX634160 IHC-Fr Image

MYH10 antibody [GT246] detects MYH10 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MYH10 stained by MYH10 antibody [GT246] (GTX634160) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX634160 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

SH Lu et al. Stem Cell Reports 2023; S2213-6711 (23) : 00184-4 Cardiac myofibrillogenesis is spatiotemporally modulated by the molecular chaperone UNC45B

Application : ICC/IF

Reactivity : Human

Yamada S et al. Bioeng Transl Med 2023; 8 (3) : e10509 Unique osteogenic profile of bone marrow stem cells stimulated in perfusion bioreactor is Rho-ROCK-mediated contractility dependent.

Fang ZM et al. Cell Prolif 2023; 56 (4) : e13386 Methyltransferase-like 3 suppresses phenotypic switching of vascular smooth muscle cells by activating autophagosome formation.

Application : WB

Reactivity : Human

He Y et al. Clin Epigenetics 2022; 14 (1) : 101 JIB-04, a histone demethylase Jumonji C domain inhibitor, regulates phenotypic switching of vascular smooth muscle cells.

Application : WB

Reactivity : Human

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