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NFIL3 antibody

Anti-NFIL3 antibody used in Western Blot (WB). GTX21078
Anti-NFIL3 antibody used in Flow cytometry (FACS). GTX21078

Cat. No. GTX21078

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human
Package
50 μg ($399)
See all NFIL3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-2μg/ml
FACS 10μg/ml

Note :

FACS
Flow cytometric analysis of Human peripheral blood mononuclear cells.
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX21078-PEP).

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RLIATQPISASDSG, from the C Terminus of the protein sequence according to NP_005375.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor, interleukin 3 regulated , E4BP4 , IL3BP1 , NF-IL3A , NFIL3A

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-NFIL3 antibody used in Western Blot (WB). GTX21078

GTX21078 WB Image

WB analysis of Jurkat nuclear lysate using GTX21078 NFIL3 antibody.
Dilution : 1μg/ml
Loading : 20μg protein in RIPA buffer

Anti-NFIL3 antibody used in Flow cytometry (FACS). GTX21078

GTX21078 FACS Image

FACS analysis of PFA fixed human peripheral blood mononuclear cells using GTX21078 NFIL3 antibody.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399