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Japanese encephalitis virus NS3 antibody

Orthogonal
Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX125868

Cat. No. GTX125868

Reference 24

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Japanese encephalitis virus
Package
100 μl ($399),
25 μl ($169)
See all Japanese encephalitis virus NS3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:2000
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

Japanese encephalitis virus infected BHK-21 cells

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Japanese encephalitis virus

Immunogen

Recombinant protein encompassing a sequence within the center region of NS3 (JEV). (Japanese Encephalitis Virus strain Jaoars982) The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177224

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Japanese encephalitis Virus Non-structural protein 3 , Japanese encephalitis Virus NS3 , JEV Non-structural protein 3 , JEV NS3

Research Area

DATA IMAGES

Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868

GTX125868 WB Image

NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by western blot analysis.
A. 10 μg BHK-21 whole cell extract
B. 10 μg whole cell extract of Japanese encephalitis virus infected BHK-21 cells
C. 10 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
10% SDS-PAGE
NS3 (Japanese encephalitis virus) antibody (GTX125868) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Japanese encephalitis virus NS3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125868

GTX125868 ICC/IF Image

NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in paraformaldehyde.
Green: NS3 (Japanese encephalitis virus) protein stained by NS3 (Japanese encephalitis virus) antibody (GTX125868) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Japanese encephalitis virus NS3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125868

GTX125868 ICC/IF Image

NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis viruswere fixed in MeOH.
Green: NS3 (Japanese encephalitis virus) protein stained by NS3 (Japanese encephalitis virus) antibody (GTX125868) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868

GTX125868 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extract (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Japanese encephalitis virus NS3 antibody (GTX125868) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868

GTX125868 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28349961

Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868

GTX125868 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28349961

Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868

GTX125868 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28349961

Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868

GTX125868 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28349961

Anti-Japanese encephalitis virus NS3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX125868

GTX125868 IHC-Fr Image

The data was published in the journal Nat Commun in 2019. PMID: 30742008

REFERENCE

Application Reference

Z Chen et al. Virol J 2023; 20 (1) : 280 Porcine promyelocytic leukemia protein isoforms suppress Japanese encephalitis virus replication in PK15 cells
Moon JH et al. BMC Neurosci 2023; 24 (1) : 59 Melatonin inhibits Japanese encephalitis virus replication and neurotoxicity via calcineurin-autophagy pathways.
Yang S et al. Front Cell Infect Microbiol 2023; 13 : 1239234 Japanese encephalitis virus perturbs PML-nuclear bodies by engaging in interactions with distinct porcine PML isoforms.
Hong JM et al. Virus Res 2023; 338 : 199249 Antiviral activity of prion protein against Japanese encephalitis virus infection in vitro and in vivo.
Liang Z et al. Viruses 2023; 15 (7) Interaction between hTIM-1 and Envelope Protein Is Important for JEV Infection.
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REVIEW

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Application Reference
Z Chen et al. Virol J 2023; 20 (1):280 Porcine promyelocytic leukemia protein isoforms suppress Japanese encephalitis virus replication in PK15 cells
Moon JH et al. BMC Neurosci 2023; 24 (1):59 Melatonin inhibits Japanese encephalitis virus replication and neurotoxicity via calcineurin-autophagy pathways.
Yang S et al. Front Cell Infect Microbiol 2023; 13:1239234 Japanese encephalitis virus perturbs PML-nuclear bodies by engaging in interactions with distinct porcine PML isoforms.
Hong JM et al. Virus Res 2023; 338:199249 Antiviral activity of prion protein against Japanese encephalitis virus infection in vitro and in vivo.
Liang Z et al. Viruses 2023; 15 (7) Interaction between hTIM-1 and Envelope Protein Is Important for JEV Infection.
Xie S et al. Microbiol Spectr 2022; 10 (4):e0114722 Japanese Encephalitis Virus (JEV) NS1' Enhances the Viral Infection of Dendritic Cells (DCs) and Macrophages in Pig Tonsils.
Chenxi Li et al. Int. J. Mol. Sci. 2022; 23 (24) A Recombinant Genotype I Japanese Encephalitis Virus Expressing a Gaussia Luciferase Gene for Antiviral Drug Screening Assay and Neutralizing Antibodies Detection
Application : WB
Xiaolong Zhou et al. Virol J 2021; 18 (1):29 Inhibition of Japanese encephalitis virus proliferation by long non-coding RNA SUSAJ1 in PK-15 cells.
Application : WB
Zhu J et al. Front Vet Sci 2021; 8:757978 Molecular Cloning of Alternative Splicing Variants of the Porcine PML Gene and Its Expression Patterns During Japanese Encephalitis Virus Infection.
Application : WB
Bhaskar M et al. mBio 2021; 12 (6):e0271221 Involvement of RIG-I Pathway in Neurotropic Virus-Induced Acute Flaccid Paralysis and Subsequent Spinal Motor Neuron Death.
Application : IHC-P
Jhan MK et al. Cells 2021; 10 (11) Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus.
Application : WB
Xie S et al. Virol Sin 2021; (Epub) Japanese Encephalitis Virus NS2B-3 Protein Complex Promotes Cell Apoptosis and Viral Particle Release by Down-Regulating the Expression of AXL.
Yu D et al. Virol Sin 2021; (Epub) C19orf66 Inhibits Japanese Encephalitis Virus Replication by Targeting -1 PRF and the NS3 Protein.
Zhao C et al. Nat Commun 2020; 11 (1):5178 CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication.
Kumar S et al. ACS Chem Neurosci 2020; 11 (21):3683-3696 Antiviral Activity of Belladonna During Japanese Encephalitis Virus Infection via Inhibition of Microglia Activation and Inflammation Leading to Neuronal Cell Survival.
Application : WB
Hsieh JT et al. Nat Commun 2019; 10 (1):706 Japanese encephalitis virus neuropenetrance is driven by mast cell chymase.
Cui X et al. Viruses 2019; 11 (6) Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro.
Application : WB
Carr M et al. Virus Genes 2019; (Epub) Upregulated expression of the antioxidant sestrin 2 identified by transcriptomic analysis of Japanese encephalitis virus-infected SH-SY5Y neuroblastoma cells.
Application : WB
Suvadip Mallick et al. bioRxiv 2019; (Epub) Novel strategy for treating neurotropic viral infections using hypolipidemic drug Atorvastatin.
Application : WB, ICC/IF
Lien JC et al. Sci Rep 2018; 8 (1):16595 Structure analysis and antiviral activity of CW-33 analogues against Japanese encephalitis virus.
Application : ICC/IF
Chen M et al. Sci Rep 2017; 7 (1):15931 Broad-spectrum antiviral agents: secreted phospholipase A2 targets viral envelope lipid bilayers derived from the endoplasmic reticulum membrane.
Lu CY et al. Viruses 2017; 9 (4) Single-Round Infectious Particle Antiviral Screening Assays for the Japanese Encephalitis Virus.
Application : ICC/IF
Xu Q et al. Sci Rep 2017; 7:45375 E3 Ubiquitin Ligase Nedd4 Promotes Japanese Encephalitis Virus Replication by Suppressing Autophagy in Human Neuroblastoma Cells.
Application : WB
Fan W et al. Sci Rep 2016; 6:33698 TRIM52 inhibits Japanese Encephalitis Virus replication by degrading the viral NS2A.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169