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PAK1 (phospho Thr212) antibody

Anti-PAK1 (phospho Thr212) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50211
Anti-PAK1 (phospho Thr212) antibody used in IHC (Paraffin sections) (IHC-P). GTX50211
Anti-PAK1 (phospho Thr212) antibody used in Western Blot (WB). GTX50211

Cat. No. GTX50211

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all PAK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 212 (P-V-T(p)-P-T) derived from human PAK1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

p21 (RAC1) activated kinase 1 , IDDMSSD , PAKalpha

Cellular Localization

Cytoplasm,Cell membrane,nucleoplasm

Background

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-PAK1 (phospho Thr212) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50211

GTX50211 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50211 PAK1 (phospho Thr212) antibody.

Anti-PAK1 (phospho Thr212) antibody used in IHC (Paraffin sections) (IHC-P). GTX50211

GTX50211 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50211 PAK1 (phospho Thr212) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-PAK1 (phospho Thr212) antibody used in Western Blot (WB). GTX50211

GTX50211 WB Image

WB analysis of extracts from 293 cells untreated or treated with forskolin using GTX50211 PAK1 (phospho Thr212) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399