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PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody

Anti-PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody used in Dot blot (Dot). GTX22477
Anti-PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody used in Western Blot (WB). GTX22477

Cat. No. GTX22477

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, Dot, ELISA

Reactivity

 Human
Package
50 μg ($399)
See all PAK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
Dot Assay dependent
ELISA 1:4000-1:20000
Not tested in other applications.

Product Note

This phospho specific polyclonal antibody is specific for phosphorylated pT423 of Human PAK 1/2/3. Reactivity with non-phosphorylated PAK 1/2/3 is less than 1% by ELISA.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human PAK 1/2/3 phospho peptide corresponding to a region of the human protein conjugated to KLH.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody used in Dot blot (Dot). GTX22477

GTX22477 Dot Image

Dot blot analysis of phosphorylated and non-phosphorylated forms of the immunizing peptide using GTX22477 PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody.
Dilution : 1:1000

Anti-PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody used in Western Blot (WB). GTX22477

GTX22477 WB Image

Western blot probing with PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody (GTX22477). The major band is at 65 kDa and weaker bands can be detected at 90 and 100 kDa. This picture was kindly submitted by Sue Thomas as part of her review.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399