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PHD1 antibody

Anti-PHD1 antibody used in Immunoprecipitation (IP). GTX64646
Anti-PHD1 antibody used in Western Blot (WB). GTX64646

Cat. No. GTX64646

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PHD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human EGLN2 (NP_444274.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

egl-9 family hypoxia inducible factor 2 , EIT-6 , EIT6 , HIF-PH1 , HIFPH1 , HPH-1 , HPH-3 , PHD1

Cellular Localization

Nucleus

Background

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-PHD1 antibody used in Immunoprecipitation (IP). GTX64646

GTX64646 IP Image

IP analysis of HeLa cell lysate using GTX64646 PHD1 antibody.
Antibody amount : 3μg / 150μg lysate
Dilution : 1:1000

Anti-PHD1 antibody used in Western Blot (WB). GTX64646

GTX64646 WB Image

WB analysis of various sample lysates using GTX64646 PHD1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399