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PHD2 antibody

Anti-PHD2 antibody used in Western Blot (WB). GTX82966
Anti-PHD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82966
Anti-PHD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82966
Anti-PHD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82966

Cat. No. GTX82966

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
25 μl ($299)
See all PHD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
ICC/IF 1:50 - 1:500
IHC-P 2.5 - 5.0 μg/ml
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A synthetic peptide made to an internal portion of mouse PHD2/HIF Prolyl Hydroxylase 2 (between residues 300-400). [UniProt# Q91YE3]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

egl-9 family hypoxia-inducible factor 1 , AI503754 , C1orf12 , HIF-PH2 , HPH-2 , Hif-p4h-2 , ORF13 , Phd2 , SM-20

Cellular Localization

Cytoplasm,Nucleus

Background

Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.

Database

Research Area

DATA IMAGES

Anti-PHD2 antibody used in Western Blot (WB). GTX82966

GTX82966 WB Image

WB analysis of mouse kidney tissue using GTX82966 PHD2 antibody.

Anti-PHD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82966

GTX82966 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX82966 PHD2 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:500

Anti-PHD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82966

GTX82966 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX82966 PHD2 antibody.
Dilution : 1:200

Anti-PHD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82966

GTX82966 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX82966 PHD2 antibody.
Dilution : 2.5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 299