Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
1:2000 |
Not tested in other applications.
Product Note
Reacts specifically with tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine. This antibody does not recognize phosphoserine or phosphothreonine.
Form
Liquid
Buffer
PBS pH7.4, 1% BSA, 50% Glycerol
Preservative
0.1% ProClin
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Immunogen
Phosphotyrosine coupled to KLH.
Purification
Purified IgG
Conjugation
Horseradish peroxidase(HRP)
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.