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Phosphotyrosine antibody [PY20] (Biotin)

Cat. No. GTX16388

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 PY20

Isotype

 IgG2b

Application

 WB, ICC/IF, ELISA, IHC

Reactivity

 Species independent
Package
100 μg ($399)
See all Phosphotyrosine products

APPLICATION

Application Note

ELISA: 1/5000. ICC: 1/500. WB: 1/5000. IHC: 1/500. Optimal dilutions/concentrations should be determined by the end user.

Product Note

Clone PY20 specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.

PROPERTIES

Form

Liquid

Buffer

0.15M Sodium Chloride, 0.02M Sodium Phosphate pH7.5, 50% Glycerol

Preservative

3mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Phosphotyrosine coupled to carrier protein

Purification

Immunogen affinity purified

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research.

Research Area

REFERENCE

Clone Reference

Valenciano AL et al. Cell Cycle 2016; 15 (20):2827-41 Extracellular-signal regulated kinase 8 of Trypanosoma brucei uniquely phosphorylates its proliferating cell nuclear antigen homolog and reveals exploitable properties.
Wang TH et al. Mol Carcinog 2015; OncomiR miR-96 and miR-182 promote cell proliferation and invasion through targeting ephrinA5 in hepatocellular carcinoma.
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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399