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Progesterone Receptor antibody [alpha PR-22]

Anti-Progesterone Receptor antibody [alpha PR-22] used in Western Blot (WB). GTX22767
Anti-Progesterone Receptor antibody [alpha PR-22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22767

Cat. No. GTX22767

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 alpha PR-22

Isotype

 IgG2b

Application

 WB, ICC/IF, IP, Gel supershift assays

Reactivity

 Human, Mouse, Rabbit, Chicken
Package
100 μg ($399)
See all Progesterone Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF 10 μg/ml
IP Assay dependent
Gel supershift assays Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Product Note

This antibody detects the A and B forms of progesterone receptor. It does not cross-react with estrogen receptor or glucocorticoid receptor.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Chicken

Immunogen

Progesterone receptor purified from chick oviduct cytosol.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

progesterone receptor , NR3C3

Cellular Localization

Nucleus,Cytoplasm

Database

Research Area

DATA IMAGES

Anti-Progesterone Receptor antibody [alpha PR-22] used in Western Blot (WB). GTX22767

GTX22767 WB Image

WB analysis of 20 ug of T47D cell lysates, untreated (-) or stimulated (+) with 100 nm promegestone for 1 hour using GTX22767 Progesterone Receptor antibody [alpha PR-22].
Dilution : 1 μg/ml

Anti-Progesterone Receptor antibody [alpha PR-22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22767

GTX22767 ICC/IF Image

ICC/IF analysis of T-47D cells using GTX22767 Progesterone Receptor antibody [alpha PR-22]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for 1 hour at room temperature

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399