RFP antibody [GT1610]

Cat. No. GTX628545

Host	Mouse
Clonality	Monoclonal
Clone Name	GT1610
Isotype	IgG2a
Application	WB, ICC/IF, IP
Reactivity	Species independent
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX628545 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IP	1:500-1:1000

Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of RFP. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888048

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX628545 IP Image

RFP tag antibody immunoprecipitates RFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 μg of RFP tag antibody (GTX628545) 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody (GTX628545) diluted at 1:1000. EasyBlot anti-nouse IgG (GTX221667-01) was used as a secondary reagent.

GTX628545 ICC/IF Image

RFP-tag antibody detects RFP-recombinant protein by immunofluorescent analysis. Sample: RFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RFP-recombinant protein stained by RFP-tag antibody (GTX628545) diluted at 1:2000. Red: RFP is expressed in the tranfected cell . Blue: Hoechst 33342 staining.

GTX628545 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody [GT1610] (GTX628545) diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

RFP antibody [GT1610]

Cat. No. GTX628545

Host

Clonality

Clone Name

Isotype

Application

Reactivity