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SEK1 / MKK4 antibody [4B4]

Anti-SEK1 / MKK4 antibody [4B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84161
Anti-SEK1 / MKK4 antibody [4B4] used in IHC (Paraffin sections) (IHC-P). GTX84161
Anti-SEK1 / MKK4 antibody [4B4] used in IHC (Paraffin sections) (IHC-P). GTX84161
Anti-SEK1 / MKK4 antibody [4B4] used in IHC (Paraffin sections) (IHC-P). GTX84161
Anti-SEK1 / MKK4 antibody [4B4] used in Western Blot (WB). GTX84161
Anti-SEK1 / MKK4 antibody [4B4] used in Immunoprecipitation (IP). GTX84161
Anti-SEK1 / MKK4 antibody [4B4] used in Flow cytometry (FACS). GTX84161
Anti-SEK1 / MKK4 antibody [4B4] used in IHC (Paraffin sections) (IHC-P). GTX84161

Cat. No. GTX84161

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4B4

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human
Package
100 μl ($519)
See all SEK1 / MKK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:150
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase 4 , JNKK , JNKK1 , MAPKK4 , MEK4 , MKK4 , PRKMK4 , SAPKK-1 , SAPKK1 , SEK1 , SERK1 , SKK1

Cellular Localization

Cytoplasm,Nucleus

Background

Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).

Database

Research Area

DATA IMAGES

Anti-SEK1 / MKK4 antibody [4B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84161

GTX84161 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SEK1 / MKK4 plasmid using GTX84161 SEK1 / MKK4 antibody [4B4].

Anti-SEK1 / MKK4 antibody [4B4] used in IHC (Paraffin sections) (IHC-P). GTX84161

GTX84161 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84161 SEK1 / MKK4 antibody [4B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-SEK1 / MKK4 antibody [4B4] used in IHC (Paraffin sections) (IHC-P). GTX84161

GTX84161 IHC-P Image

IHC-P analysis of human colon tissue using GTX84161 SEK1 / MKK4 antibody [4B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-SEK1 / MKK4 antibody [4B4] used in IHC (Paraffin sections) (IHC-P). GTX84161

GTX84161 IHC-P Image

IHC-P analysis of human breast tissue using GTX84161 SEK1 / MKK4 antibody [4B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-SEK1 / MKK4 antibody [4B4] used in Western Blot (WB). GTX84161

GTX84161 WB Image

WB analysis of HEK293T cells transfected with SEK1 / MKK4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84161 SEK1 / MKK4 antibody [4B4].
Loading : 5 ug per lane

Anti-SEK1 / MKK4 antibody [4B4] used in Immunoprecipitation (IP). GTX84161

GTX84161 IP Image

IP analysis of DDDDK tagged SEK1 / MKK4 overexpressed HEK293T lysate using GTX84161 SEK1 / MKK4 antibody [4B4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-SEK1 / MKK4 antibody [4B4] used in Flow cytometry (FACS). GTX84161

GTX84161 FACS Image

FACS analysis of HEK293T cells transfected with either SEK1 / MKK4 plasmid(Red) or empty vector control plasmid(Blue) using GTX84161 SEK1 / MKK4 antibody [4B4].

Anti-SEK1 / MKK4 antibody [4B4] used in IHC (Paraffin sections) (IHC-P). GTX84161

GTX84161 IHC-P Image

IHC-P analysis of human lung tissue using GTX84161 SEK1 / MKK4 antibody [4B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519