APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:1000 |
| 1:100-1:200 |
| 1:50-1:100 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide sequence around phosphorylation site of serine 80 (T-H-S(p)-I-E) derived from human SEK1 / MKK4.
Purification
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase kinase 4 , JNKK , JNKK1 , MAPKK4 , MEK4 , MKK4 , PRKMK4 , SAPKK-1 , SAPKK1 , SEK1 , SERK1 , SKK1
Cellular Localization
Cytoplasm,Nucleus
Background
This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Database
Research Area
DATA IMAGES
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GTX50227 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX50227 SEK1 / MKK4 (phospho Ser80) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
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GTX50227 ICC/IF Image
ICC/IF analysis of methanol-fixed HeLa cells using GTX50227 SEK1 / MKK4 (phospho Ser80) antibody.
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GTX50227 WB Image
WB analysis of extracts from HepG2 cells untreated or treated with EGF using GTX50227 SEK1 / MKK4 (phospho Ser80) antibody.
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REFERENCE
There are currently no references for SEK1 / MKK4 (phospho Ser80) antibody (GTX50227). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SEK1 / MKK4 (phospho Ser80) antibody (GTX50227). Be the first to share your experience with this product.
