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TGF beta 1 antibody

Anti-TGF beta 1 antibody used in ELISA (ELISA). GTX29758
Anti-TGF beta 1 antibody used in Western Blot (WB). GTX29758
Anti-TGF beta 1 antibody used in Western Blot (WB). GTX29758
Anti-TGF beta 1 antibody used in Western Blot (WB). GTX29758
Anti-TGF beta 1 antibody used in ELISA (ELISA). GTX29758
Anti-TGF beta 1 antibody used in Western Blot (WB). GTX29758

Cat. No. GTX29758

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
50 μg ($399)
See all TGF beta 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:500
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA 1:10000-1:20000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino acids near the amino terminus of the mature growth factor (112 amino acids in length).

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transforming growth factor beta 1 , CED , DPD1 , IBDIMDE , LAP , TGFB , TGFbeta

Cellular Localization

Secreted,Extracellular space,Extracellular matrix

Background

Transforming Growth Factor Beta 1 is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non-neoplastic tissues, release transforming growth factors; and essentially all cells possess a specific TGF beta 1 receptor. The multi-modal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function.

Database

Research Area

DATA IMAGES

Anti-TGF beta 1 antibody used in ELISA (ELISA). GTX29758

GTX29758 ELISA Image

ELISA analysis of SA-conjugated immunizing peptide using GTX29758 TGF beta 1 antibody. Each well was coated in duplicate with 0.1μg of conjugate.

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX29758

GTX29758 WB Image

WB analysis of recombinant TGF Beta 1 proteins using GTX29758 TGF beta 1 antibody.
Lane 1 : Reduced recombinant TGF Beta 1
Lane 2 : Protein Ladder
Lane 3 : Non-reduced recombinant TGF Beta 1
Loading : 250ng per lane
Dilution : 1:200

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX29758

GTX29758 WB Image

Western blot analysis using GeneTex's Affinity Purified anti-TGF beta 1 antibody to detect human TGF beta 1. Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). Comparison to molecular weight markers (not shown) was used to estimate the indicated molecular weights. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 1 h followed by detection using IRDyeR 800 labeled Goat-a-Rabbit IgG (H&L) diluted 1:2,500. IRDyeR 800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX29758

GTX29758 WB Image

WB analysis of human TGF beta 1 protein using GTX29758 TGF beta 1 antibody.
Lane 1 : Non-reduced TGF beta 1 protein
Lane 2 : Reduced TGF beta 1 protein
Loading : 250 ng
Dilution : 1:200

Anti-TGF beta 1 antibody used in ELISA (ELISA). GTX29758

GTX29758 ELISA Image

ELISA analysis of BSA-conjugated immunizing peptide using serially diluted GTX29758 TGF beta 1 antibody.
Coating : 0.1 μg

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX29758

GTX29758 WB Image

WB analysis of human TGF beta 1 recombinant protein using GTX29758 TGF beta 1 antibody.
Lane 1 : Reduced TGF beta 1 protein
Lane 2 : Protein ladder
Lane 3 : Non-reduced TGF beta 1 protein

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399