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beta Tubulin antibody [2-28-33]

Anti-beta Tubulin antibody [2-28-33] used in Western Blot (WB). GTX11311

Cat. No. GTX11311

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2-28-33

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Rat, Bovine, Chicken, Caenorhabditis elegans, Ciona intestinalis, Strongylocentrotus purpuratus
Package
200 μl ($829)
See all beta Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100
ICC/IF 1:1,000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

chicken fibroblasts, human foreskin fibroblasts

Product Note

The antibody recognizes the two major and one of the minor β‑tubulin isotypes of the free-living soil nematode C. elegans. It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, to neuronal axons of the larva of an ascidian (Ciona intestinalis) and to tubulin of chicken and mammals, cultured human fibroblasts, bovine and rat brain tissue. In C.elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and β-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels. It reacts with heat-denatured and reduced mammalian preparations in immunoblotting.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Sea Urchin

Immunogen

sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin beta class I , CDCBM6 , CSCSC1 , M40 , OK/SW-cl.56 , TUBB1 , TUBB5

Cellular Localization

Cytoplasm, cytoskeleton

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Database

Research Area

DATA IMAGES

Anti-beta Tubulin antibody [2-28-33] used in Western Blot (WB). GTX11311

GTX11311 WB Image

WB analysis of chicken fibroblasts extract using GTX11311 beta Tubulin antibody [2-28-33].
Lane A : Antibody dilution 1:200
Lane B : Negative control (only secondary antibody)

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 829