|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||30 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , NIH-3T3 , PC-12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Product Description||Rabbit Polyclonal antibody to Prohibitin (prohibitin)|
|Background||Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq]|
|Synonyms||PHB1 antibody, PHB antibody, prohibitin antibody|
|Cellular Localization||Mitochondrion inner membrane|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Dog, Cat, Chicken, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Zebrafish (93%), Xenopus laevis (92%), Dog (100%), Cat (100%), Chicken (95%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%)|