GeneTex
  • Country / Location Selection

United States (US)

RANTES antibody [53405.111]

Cat. No. GTX10394

Host

Rat

Clonality

Monoclonal

Clone Name

53405.111

Isotype

IgG2a

Application

ICC/IF, Neutralizing/Inhibition

Reactivity

Mouse
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 8-25 μg/ml
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

10 kDa. ( Note )

Positive Control

mouse splenocytes

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 5% Trehalose

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

purified, E. coli-derived, recombinant mouse RANTES.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chemokine (C-C motif) ligand 5 , MuRantes , RANTES , SISd , Scya5 , TCP228

Cellular Localization

Secreted

Background

RANTES is a member of the beta (C-C) chemokine subfamily, which also includes the human proteins MCP1, MCP2, MIP1 alpha, MIP1 beta, I309, and their murine counterparts. Mouse RANTES cDNA encodes a 91 amino acid residue precursor polypeptide with a 23 amino acid residue hydrophobic signal peptide that is cleaved to produce the 68 amino acid residue mature protein (approximately 7.9 kDa). Human and mouse RANTES share approximately 85 % amino acid identity. RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) was first discovered by subtractive hybridization as a transcript expressed in T cells but not B cells. Eosinophillic chemotactic substances released by thrombin stimulated human platelets is identical to RANTES. In addition to T cells and platelets, RANTES is expressed by renal tubular epithelium , synovial fibroblasts, and selected tumor cells (rhabdomyosarcoma cells and MG63 osteosarcoma cells). The mouse RANTES gene maps to chromosome 11. The chemokine RANTES is a chemoattractant for monocytes, T lymphocytes , and NK (natural killer) cells. RANTES selectively attracts T cells of the CD4+/CD45RO+ phenotype in vitro. RANTES can also chemoattract and degranulate eosinophils , as well as chemoattract and induce histamine release from basophils. RANTES has been shown to inhibit HIV-1 entry via human CCR5. RANTES and MIP alpha bind to chemokine receptors (CCR1, CCR4, and CCR5) and induce activation of STAT signaling pathways. Because of its in vitro biological activities, RANTES may play an important role in mediating immune and inflammatory responses.

Database

Research Area

REFERENCE

There are currently no references for RANTES antibody [53405.111] (GTX10394). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RANTES antibody [53405.111] (GTX10394). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 399