RFC4 antibody [N1C3]
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||40 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse liver|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||replication factor C (activator 1) 4, 37kDa|
|Product Description||Rabbit Polyclonal antibody to RFC4 (replication factor C (activator 1) 4, 37kDa)|
|Background||The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]|
|Synonyms||A1 antibody, MGC27291 antibody, RFC37 antibody, RFC4 antibody, A1 37 kDa subunit antibody, activator 1 37 kDa subunit antibody, RF-C 37 kDa subunit antibody, activator 1 subunit 4 antibody, replication factor C 37 kDa subunit antibody, RFC 37 kDa subunit antibody, replication factor C subunit 4 antibody, "replication factor C (activator 1) 4, 37kDa antibody"|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human RFC4. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Xenopus laevis, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rat (92%), Xenopus laevis (84%), Chicken (86%), Rhesus Monkey (99%), Bovine (86%)|