ROCK1 antibody [GT464]
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||293T , HeLa , HepG2 , Neuro2A , GL261 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , PC-12 (30uM cisplatin for 24hr)|
(Please refer to the vial label for the specific concentration)|
|Purification||Affinity purified by Protein G.|
|Full Name||Rho-associated, coiled-coil containing protein kinase 1|
|Product Description||Mouse Monoclonal antibody [GT464] to ROCK1 (Rho-associated, coiled-coil containing protein kinase 1)|
|Background||This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]|
|Synonyms||P160ROCK Antibody, Rho-associated, coiled-coil containing protein kinase 1 Antibody, ROCK-I Antibody|
|Cellular Localization||Cytoplasm , Centrosome , centriole (By similarity) , Golgi apparatus membrane; Peripheral membrane protein |
|Immunogen||Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Rabbit, Chicken, Rhesus Monkey|
|Predict Reactivity Note||Zebrafish (90%), Rabbit (98%), Chicken (96%), Rhesus Monkey (98%)|