ROCK1 antibody [N1N2], N-term
|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||158 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , NIH-3T3 , C2Cl2 , rat brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||Rho-associated, coiled-coil containing protein kinase 1|
|Product Description||Rabbit Polyclonal antibody to ROCK1 (Rho-associated, coiled-coil containing protein kinase 1)|
|Background||This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]|
|Synonyms||MGC131603 antibody, MGC43611 antibody, P160ROCK antibody, PRO0435 antibody, ROCK1 antibody, p160 ROCK-1 antibody, rho-associated, coiled-coil-containing protein kinase 1 antibody, rho-associated protein kinase 1 antibody, renal carcinoma antigen NY-REN-35 antibody, rho-associated, coiled-coil-containing protein kinase I antibody, Rho kinase antibody, p160-ROCK antibody, Rho-associated, coiled-coil containing protein kinase 1 antibody|
|Cellular Localization||Cytoplasm , Centrosome , centriole (By similarity) , Golgi apparatus membrane; Peripheral membrane protein |
|Immunogen||Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Rabbit, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Zebrafish (90%), Rabbit (98%), Chicken (96%), Rhesus Monkey (98%), Bovine (99%)|