|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||161 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , Rat2 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , A549 , H1299 , HCT116|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||Rho-associated, coiled-coil containing protein kinase 2|
|Product Description||Rabbit Polyclonal antibody to ROCK2 (Rho-associated, coiled-coil containing protein kinase 2)|
|Background||The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]|
|Synonyms||Rho-associated, coiled-coil containing protein kinase 2 Antibody, ROCK-II Antibody|
|Cellular Localization||Cytoplasm (By similarity) , Cell membrane; Peripheral membrane protein (By similarity) |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Xenopus laevis (80%), Rhesus Monkey (100%), Bovine (97%)|