ROCK2 antibody [N3C1], Internal

Name: ROCK2 antibody [N3C1], Internal
Brand: GeneTex
SKU: GTX108247
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-ROCK2 antibody [N3C1], Internal used in Western Blot (WB). GTX108247

Anti-ROCK2 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108247

Anti-ROCK2 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX108247

Cat. No. GTX108247

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all ROCK2 products

View product citations for antibody GTX108247 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Positive Control

NIH-3T3 , JC , Rat brain , H1299 , HeLa , HepG2 , 293T

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037903

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 2 , ROCK-II

Cellular Localization

Cytoplasm , Cell membrane; Peripheral membrane protein

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]

Database

Gene ID: 9475 ROCK2
UniProt: O75116 ROCK2

Research Area

DATA IMAGES

GTX108247 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [N3C1], Internal (GTX108247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108247 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody [N3C1], Internal (GTX108247) diluted at 1:500 and competitor's antibody (sc-1851) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX108247 WB Image

Sample (50 μg of whole cell lysate)
A: Rat brain
5% SDS PAGE
GTX108247 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108247 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using ROCK2(GTX108247) antibody at 1:200 dilution.

GTX108247 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
5% SDS PAGE
GTX108247 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108247 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
5% SDS PAGE
GTX108247 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108247 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human esophagous cancer, using ROCK2(GTX108247) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Fujimura K et al. J Biol Chem 2015; 290 (50) : 29907-19 Eukaryotic Translation Initiation Factor 5A (EIF5A) Regulates Pancreatic Cancer Metastasis by Modulating RhoA and Rho-associated Kinase (ROCK) Protein Expression Levels.

Chou HC et al. Arch Biochem Biophys 2014; 543 : 15-22 5-Methoxytryptophan-dependent protection of cardiomyocytes from heart ischemia reperfusion injury.

Application : WB

Reactivity : Rat

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