RPA70 antibody [C1C3]
|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||68 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , Mouse liver|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||replication protein A1, 70kDa|
|Product Description||Rabbit Polyclonal antibody to RPA70 (replication protein A1, 70kDa)|
|Background||Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.|
|Synonyms||HSSB antibody, MST075 antibody, REPA1 antibody, RF-A antibody, RP-A antibody, RPA70 antibody, RPA1 antibody, single-stranded DNA-binding protein antibody, replication protein A 70 kDa DNA-binding subunit antibody, replication factor A protein 1 antibody, RP-A p70 antibody, RF-A protein 1 antibody, MSTP075 antibody, "replication protein A1, 70kDa antibody"|
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human RPA70. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Sheep, Bovine|
|Predict Reactivity Note||Rat (87%), Sheep (93%), Bovine (93%)|