|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||34 kDa (note)
|Positive Controls||A431 , H1299 , HeLaS3 , Molt-4 , mouse brain , Rat2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||ribosomal protein L5|
|Product Description||Rabbit Polyclonal antibody to RPL5 (ribosomal protein L5)|
|Background||Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]|
|Synonyms||DBA6 antibody, MGC117339 antibody, RPL5 antibody, 60S ribosomal protein L5 antibody, ribosomal protein L5 antibody|
|Cellular Localization||Cytoplasm , Nucleus , nucleolus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human RPL5. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Pig, Rabbit, Chicken, Bovine|
|Predict Reactivity Note||Xenopus laevis (82%), Pig (97%), Rabbit (98%), Chicken (90%), Bovine (98%)|