|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||30 kDa (note)
|Positive Controls||A431 , Jurkat , Raji , Neuro2A , GL261 , C8D30 , PC-12 , Rat2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||ribosomal protein L7a|
|Product Description||Rabbit Polyclonal antibody to RPL7A (ribosomal protein L7a)|
|Background||Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ribosomal proteins. It can interact with a subclass of nuclear hormone receptors, including thyroid hormone receptor, and inhibit their ability to transactivate by preventing their binding to their DNA response elements. This gene is included in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. It is co-transcribed with the U24, U36a, U36b, and U36c small nucleolar RNA genes, which are located in its second, fifth, fourth, and sixth introns, respectively. This gene rearranges with the trk proto-oncogene to form the chimeric oncogene trk-2h, which encodes an oncoprotein consisting of the N terminus of ribosomal protein L7a fused to the receptor tyrosine kinase domain of trk. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]|
|Synonyms||SURF3 antibody, TRUP antibody, RPL7A antibody, surfeit locus protein 3 antibody, PLA-X polypeptide antibody, surfeit 3 antibody, thyroid hormone receptor uncoupling protein antibody, 60S ribosomal protein L7a antibody, ribosomal protein L7a antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human RPL7A. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Dog, Chicken, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Zebrafish (93%), Xenopus laevis (93%), Dog (100%), Chicken (95%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%)|