|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
| Immunoprecipitation||Assay-dependent dilution
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||30 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||ribosomal protein S3A|
|Product Description||Rabbit Polyclonal antibody to RPS3A (ribosomal protein S3A)|
|Background||Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing alternative transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]|
|Synonyms||FTE1 antibody, MFTL antibody, MGC23240 antibody, RPS3A antibody, 40S ribosomal protein S3a antibody, v-fos transformation effector protein 1 antibody, fte-1 antibody, ribosomal protein S3A antibody|
|Specificity||Knockdown/Knockout validation was supported by references (PMID:26990996)|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human RPS3A. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Japanese Medaka, Xenopus laevis, Pig, Chicken, Bovine|
|Predict Reactivity Note||Zebrafish (93%), Japanese Medaka (93%), Xenopus laevis (96%), Pig (97%), Chicken (96%), Bovine (100%)|