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1 .GTX105020 RPS3A antibody

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ECL kit RIPA Extraction Kit Protein Ladder

RPS3A antibody

Knockout/Knockdown

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, IP, WB
  • Species reactivityHuman, Mouse, Rat
  • Publication  ( 1 )
Cat No: GTX105020
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-RPS3A antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX105020
  • Anti-RPS3A antibody used in Western Blot (WB). GTX105020
  • Anti-RPS3A antibody used in Western Blot (WB). GTX105020
  • Anti-RPS3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105020
  • Anti-RPS3A antibody used in Western Blot (WB). GTX105020
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  • Datasheet
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    Reference (1)
  • Bio-info
  • Protocol & MSDS
  • Application Information: RPS3A antibody

Catalog Number GTX105020
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( 0 )
Publication ( 1 )
Product Name RPS3A antibody
See all RPS3A products
ApplicationsICC/IF, IHC-P, IP, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
ImmunoprecipitationAssay-dependent dilution  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size30 kDa (note)
Positive Controls293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: RPS3A antibody

Full Nameribosomal protein S3A
BackgroundRibosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing alternative transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]
SynonymsFTE1 antibody, MFTL antibody, MGC23240 antibody, RPS3A antibody, 40S ribosomal protein S3a antibody, v-fos transformation effector protein 1 antibody, fte-1 antibody, ribosomal protein S3A antibody
Validated NoteKnockdown/Knockout validation was supported by references (PMID:26990993)
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human RPS3A. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Zebrafish, Japanese Medaka, Xenopus laevis, Pig, Chicken, Bovine
Predict Reactivity NoteZebrafish (93%), Japanese Medaka (93%), Xenopus laevis (96%), Pig (97%), Chicken (96%), Bovine (100%)
ConjugationUnconjugated

  • Storage Conditions: RPS3A antibody

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
RPS3A antibody validated data
  • Anti-RPS3A antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX105020
    • GTX105020 IHC-P Image
      RPS3A antibody detects RPS3A protein at cytosol on mouse brain stem by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse brain stem.
      RPS3A antibody (GTX105020) dilution: 1:200.
      Top
  • Anti-RPS3A antibody used in Western Blot (WB). GTX105020
    • GTX105020 WB Image
      RPS3A antibody detects RPS3A protein by Western blot analysis.
      A. 30 µg PC-12 whole cell lysate/extract
      B. 30 µg Rat2 whole cell lysate/extract
      12 % SDS-PAGE
      RPS3A antibody (GTX105020) dilution: 1:1000
      Top
  • Anti-RPS3A antibody used in Western Blot (WB). GTX105020
    • GTX105020 WB Image
      RPS3A antibody detects RPS3A protein by Western blot analysis.
      A. 30 µg Neuro2A whole cell lysate/extract
      B. 30 µg GL261 whole cell lysate/extract
      C. 30 µg C8D30 whole cell lysate/extract
      D. 30 µg NIH-3T3 whole cell lysate/extract
      E. 30 µg BCL-1 whole cell lysate/extract
      F. 30 µg Raw264.7 whole cell lysate/extract
      G. 30 µg C2C12 whole cell lysate/extract
      12 % SDS-PAGE
      RPS3A antibody (GTX105020) dilution: 1:1000
      Top
  • Anti-RPS3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105020
    • GTX105020 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ribosomal protein S3a(GTX105020) antibody at 1:200 dilution.
      Top
  • Anti-RPS3A antibody used in Western Blot (WB). GTX105020
    • GTX105020 WB Image
      Sample(30 ug whole cell lysate)
      A: 293T
      B: A431 (GTX27909)
      C: H1299
      D: HeLa S3 (GTX14654)
      E: Hep G2 (GTX27900)
      F: MOLT4 (GTX27912)
      G: Raji (GTX27908)
      12% SDS PAGE
      GTX105020 diluted at 1:1000
      Top
  • Anti-RPS3A antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX105020
    • GTX105020 IHC-P Image
      RPS3A antibody detects RPS3A protein at cytosol on mouse brain stem by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse brain stem.
      RPS3A antibody (GTX105020) dilution: 1:200.
      Top
  • Anti-RPS3A antibody used in Western Blot (WB). GTX105020
    • GTX105020 WB Image
      RPS3A antibody detects RPS3A protein by Western blot analysis.
      A. 30 µg PC-12 whole cell lysate/extract
      B. 30 µg Rat2 whole cell lysate/extract
      12 % SDS-PAGE
      RPS3A antibody (GTX105020) dilution: 1:1000
      Top
  • Anti-RPS3A antibody used in Western Blot (WB). GTX105020
    • GTX105020 WB Image
      RPS3A antibody detects RPS3A protein by Western blot analysis.
      A. 30 µg Neuro2A whole cell lysate/extract
      B. 30 µg GL261 whole cell lysate/extract
      C. 30 µg C8D30 whole cell lysate/extract
      D. 30 µg NIH-3T3 whole cell lysate/extract
      E. 30 µg BCL-1 whole cell lysate/extract
      F. 30 µg Raw264.7 whole cell lysate/extract
      G. 30 µg C2C12 whole cell lysate/extract
      12 % SDS-PAGE
      RPS3A antibody (GTX105020) dilution: 1:1000
      Top
  • Anti-RPS3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105020
    • GTX105020 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ribosomal protein S3a(GTX105020) antibody at 1:200 dilution.
      Top
  • Anti-RPS3A antibody used in Western Blot (WB). GTX105020
    • GTX105020 WB Image
      Sample(30 ug whole cell lysate)
      A: 293T
      B: A431 (GTX27909)
      C: H1299
      D: HeLa S3 (GTX14654)
      E: Hep G2 (GTX27900)
      F: MOLT4 (GTX27912)
      G: Raji (GTX27908)
      12% SDS PAGE
      GTX105020 diluted at 1:1000
      Top
Back to Top
  • Reference for RPS3A antibody
Application References Application Reactivity
Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell 61 (6):903-13 (2016). WB, ICC/IF, IP Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensRPS3A6189B2R4D4, P61247180478NM_001006NP_00099729.95

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Rattus norvegicusRps3a29288P49242NM_017153NP_05884929.95
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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