|Applications||ICC/IF, IHC-P, WB, IHC-Wm|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
| IHC (Whole mount)||Assay-dependent dilution
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||29 kDa (note)
|Positive Controls||HeLa , HepG2 , Mouse brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||ribosomal protein S6|
|Product Description||Rabbit Polyclonal antibody to RPS6 (ribosomal protein S6)|
|Background||Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]|
|Synonyms||RPS6 antibody, 40S ribosomal protein S6 antibody, phosphoprotein NP33 antibody, ribosomal protein S6 antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human RPS6. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Zebrafish|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Drosophila, Dog, Chicken, Bovine|
|Predict Reactivity Note||Rat (100%), Drosophila (84%), Dog (100%), Chicken (98%), Bovine (100%)|