Rabbit IgM antibody
|Full Name||Goat anti-Rabbit IgM polyclonal antibody|
|Synonyms||Rabbit IgM secondary antibody, Goat anti-Rabbit IgM, antiRabbit IgM antibody, Goat antiRabbit IgM, anti-Rabbit IgM antibody, Goat anti Rabbit IgM, anti Rabbit IgM antibody|
|Product Description||Goat anti-Rabbit IgM Polyclonal antibody|
|Specificity||By immunoelectrophoresis and ELISA this antibodyreacts specifically with rabbit IgM. This antibody is specific for the ì chain of IgM.|
|Background||IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenstres are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.|
|Applications||ELISA, ICC/IF, IHC, WB|
|Application Note||Recommended Starting Dilutions:|
ELISA (Coating): Use at a dilution of 1:100 - 1:500. ELISA (Primary): Use at a dilution of 1:1,000 - 1:30,000.
ICC/IF: Use at a dilution of 1:200 - 1:2,000.
IHC: Use at a dilution of 1:200 - 1:2,000. WB (Colorimetric): Use at a dilution of 1:1,000 - 1:10,000. WB (Chemiluminescent): Use at a dilution of 1:1,000 - 1:30,000.
Not tested in other applications
Optimal dilutions/concentrations should be determined by the researcher.
|Purification||Immunogen affinity purified|
(Please refer to the vial label for the specific concentration)|
|Storage Buffer||Phosphate-buffered saline, pH 7.2, containing 0.1% sodium azide|
|Storage Instruction||Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.|
|Notes||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.|