Rabbit IgM antibody (HRP)
|Full Name||HRP-conjugated Goat anti-Rabbit IgM polyclonal antibody|
|Synonyms||Goat antiRabbit IgM HRP, Rabbit IgM secondary antibody, antiRabbit IgM antibody, anti-Rabbit IgM antibody, Goat anti-Rabbit IgM HRP, anti Rabbit IgM antibody, Goat anti Rabbit IgM HRP|
|Product Description||HRP-conjugated Goat anti-Rabbit IgM Polyclonal antibody|
|Specificity||By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgM. This antibody is specific for the ì chain of IgM.|
|Background||IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenstres are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.|
|Applications||ELISA, ICC/IF, WB|
|Application Note||Recommended Starting Dilutions:|
ELISA: Use at a dilution of 1/10,000 - 1/100,000.
ICC/IF: Use at a dilution of 1/200 - 1/500.
WB:Colorimetric - Use at a dilution of 1/1,000 - 1/10,000. Chemiluminescent - Use at a dilution of 1/1,000-1/30,000 Optimal dilutions/concentrations should be determined by the end user.
|Purification||Immunogen affinity purified|
|Purification Note||Antiserum was solid phase adsorbed to ensure classspecificity. The antibody was isolated by affinitychromatography using antigen coupled to agarose beadsand conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinctioncoe|
(Please refer to the vial label for the specific concentration)|
|Storage Buffer||50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal|
|Storage Instruction||Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.|
|Notes||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.|