Rad50 antibody [N1N2], N-term
|Applications||IHC-P, IP, WB, IB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
| Immunoblotting||Assay-dependent dilution
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||154 kDa (note)
|Positive Controls||HeLa , NT2D1 , Neuro2A , GL261 , PC-12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||RAD50 homolog (S. cerevisiae)|
|Product Description||Rabbit Polyclonal antibody to Rad50 (RAD50 homolog (S. cerevisiae))|
|Background||The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq]|
|Synonyms||NBSLD antibody, RAD502 antibody, hRad50 antibody, RAD50 antibody, DNA repair protein RAD50 antibody, RAD50 homolog (S. cerevisiae) antibody|
|Cellular Localization||Nucleus , Telomere |
|Immunogen||Recombinant protein encompassing a sequence within the N-terminus region of human Rad50. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Rabbit (92%), Bovine (93%)|