Rad51 antibody [N1C2]
|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||37 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)|
|Product Description||Rabbit Polyclonal antibody to Rad51 (RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae))|
|Background||The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist. [provided by RefSeq]|
|Synonyms||BRCC5 antibody, HRAD51 antibody, HsRad51 antibody, HsT16930 antibody, RAD51A antibody, RECA antibody, RAD51 antibody, "BRCA1/BRCA2-containing complex, subunit 5 antibody", DNA repair protein RAD51 homolog 1 antibody, "RecA, E. coli, homolog of antibody", "RAD51 homolog (RecA homolog, E. coli) antibody", recombination protein A antibody, RAD51 homolog A antibody, RecA-like protein antibody, "RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody"|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Rad51. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Zebrafish, Dog, Pig, Rabbit, Chicken, Bovine|
|Predict Reactivity Note||Rat (98%), Zebrafish (90%), Dog (98%), Pig (99%), Rabbit (97%), Chicken (95%), Bovine (98%)|