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1 .GTX60556 Raf1 antibody [4G4]

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Raf1 antibody [4G4]


  • Host / ClonalityMouse Monoclonal
  • Clone Name4G4
  • ApplicationsELISA, FACS, IHC-P, WB
  • Species reactivityHuman, Monkey, Rat
Cat No: GTX60556
Package Price Qty
100 μgUSD 319.00
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  • Anti-Raf1 antibody [4G4] used in ELISA. GTX60556
  • Anti-Raf1 antibody [4G4] used in Flow cytometry/FACS. GTX60556
  • Anti-Raf1 antibody [4G4] used in IHC (Formalin-fixed paraffin-embedded sections). GTX60556
  • Anti-Raf1 antibody [4G4] used in Western Blot (WB). GTX60556
  • Anti-Raf1 antibody [4G4] used in Western Blot (WB). GTX60556
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  • Datasheet
  • Application Information: Raf1 antibody [4G4]

Catalog Number GTX60556
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Product Name Raf1 antibody [4G4]
See all Raf1 products
ApplicationsELISA, FACS, IHC-P, WB
Application Note
Suggested dilutionReference
ELISA1/10000*  
Flow cytometry/FACS1/200 - 1/400*  
IHC (Formalin-fixed paraffin-embedded sections)1/200 - 1/1000*  
Western Blot (WB)1/500 - 1/2000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationProtein G Purified

  • Specifications: Raf1 antibody [4G4]

Full NameRaf-1 proto-oncogene, serine/threonine kinase
BackgroundThis gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]
SynonymsCRAF Antibody , RAF1 Antibody , NS5 Antibody , C RAF Antibody
HostMouse
ClonalityMonoclonal
Clone Name4G4
IsotypeIgG1
ImmunogenPurified recombinant fragment of human RAF1 expressed in E. Coli.
Antigen SpeciesHuman
Species ReactivityHuman, Monkey, Rat
ConjugationUnconjugated

  • Storage Conditions: Raf1 antibody [4G4]

Storage BufferPBS, 0.05% sodium azide
Storage InstructionKeep as concentrated solution. For short-term storage, store at 4° C (up to 10 days). For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Type of cancer > Prostate cancer
  • Cancer > Type of cancer > Thyroid
  • Disease Related > Cardiovascular > Heart > Heart disease
  • Signal Transduction > Signaling through enzyme-linked cell surface receptors > PI 3-Kinase
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Raf1 antibody [4G4] validated data
  • Anti-Raf1 antibody [4G4] used in ELISA. GTX60556
    • GTX60556 ELISA Image
      ELISA analysis of antigen using GTX60556 Raf1 antibody [4G4].
      Black : Control antigen 100ng
      Purple : Antigen 10ng
      Blue : Antigen 50ng
      Red : Antigen 100ng
      Top
  • Anti-Raf1 antibody [4G4] used in Flow cytometry/FACS. GTX60556
    • GTX60556 FACS Image
      FACS analysis of HeLa cells using GTX60556 Raf1 antibody [4G4].
      Green : Raf1
      Red : negative control
      Top
  • Anti-Raf1 antibody [4G4] used in IHC (Formalin-fixed paraffin-embedded sections). GTX60556
    • GTX60556 IHC-P Image
      IHC-P analysis of liver cancer tissue using GTX60556 Raf1 antibody [4G4].
      Top
  • Anti-Raf1 antibody [4G4] used in Western Blot (WB). GTX60556
    • GTX60556 WB Image
      WB analysis of HeLa (1), A431 (2), Cos7 (3) and C6 (4) cell lysate using GTX60556 Raf1 antibody [4G4].
      Top
  • Anti-Raf1 antibody [4G4] used in Western Blot (WB). GTX60556
    • GTX60556 WB Image
      WB analysis of human RAF1 (AA: 198-407) recombinant protein using GTX60556 Raf1 antibody [4G4].
      Top
  • Anti-Raf1 antibody [4G4] used in ELISA. GTX60556
    • GTX60556 ELISA Image
      ELISA analysis of antigen using GTX60556 Raf1 antibody [4G4].
      Black : Control antigen 100ng
      Purple : Antigen 10ng
      Blue : Antigen 50ng
      Red : Antigen 100ng
      Top
  • Anti-Raf1 antibody [4G4] used in Flow cytometry/FACS. GTX60556
    • GTX60556 FACS Image
      FACS analysis of HeLa cells using GTX60556 Raf1 antibody [4G4].
      Green : Raf1
      Red : negative control
      Top
  • Anti-Raf1 antibody [4G4] used in IHC (Formalin-fixed paraffin-embedded sections). GTX60556
    • GTX60556 IHC-P Image
      IHC-P analysis of liver cancer tissue using GTX60556 Raf1 antibody [4G4].
      Top
  • Anti-Raf1 antibody [4G4] used in Western Blot (WB). GTX60556
    • GTX60556 WB Image
      WB analysis of HeLa (1), A431 (2), Cos7 (3) and C6 (4) cell lysate using GTX60556 Raf1 antibody [4G4].
      Top
  • Anti-Raf1 antibody [4G4] used in Western Blot (WB). GTX60556
    • GTX60556 WB Image
      WB analysis of human RAF1 (AA: 198-407) recombinant protein using GTX60556 Raf1 antibody [4G4].
      Top
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