Raf1 antibody [N1C1-2]
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||73 kDa (note)
|Positive Controls||A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||v-raf-1 murine leukemia viral oncogene homolog 1|
|Product Description||Rabbit Polyclonal antibody to RAF1 (v-raf-1 murine leukemia viral oncogene homolog 1)|
|Background||This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq]|
|Synonyms||CRAF antibody, NS5 antibody, Raf-1 antibody, c-Raf antibody, RAF1 antibody, proto-oncogene c-RAF antibody, raf proto-oncogene serine/threonine protein kinase antibody, RAF proto-oncogene serine/threonine-protein kinase antibody, Oncogene RAF1 antibody, v-raf-1 murine leukemia viral oncogene homolog 1 antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Raf1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rat (97%), Chicken (92%), Rhesus Monkey (100%), Bovine (95%)|