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1 .GTX27908 Raji cell lysate

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Raji cell lysate


  • ApplicationsWB
Cat No: GTX27908
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100 μgUSD 199.00
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  • Application Information: Raji cell lysate

Catalog Number GTX27908
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Product Name Raji cell lysate
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BackgroundRaji cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetraacetic acid, 1 mM phenyl methyl sulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Lysate FractionWhole Cell Extract/Total Protein
Cell LinesRaji
ApplicationsWB
Application NoteRaji cell lysate is ready to load on SDS-PAGE for Western blotting, 20 μg per lane isrecommended for mini gel.
Form SuppliedLiquid
Concentration2 mg/ml
Storage BufferSDS sample buffer with 5% β-mercaptoethanol.
Storage InstructionStore at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -20˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Raji cell lysate validated data
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All products are for research use only—Not for use in diagnostic procedures.