Rat IgG (F(ab')2) antibody, F(ab')2 fragment, pre-adsorbed (HRP)
|Product Description||HRP-conjugated Goat anti-Rat IgG (F(ab')2) Polyclonal antibody, F(ab')2 fragment, pre-adsorbed|
|Specificity||This antibody has been pre-adsorbed against Bovine, Horse and Human serum proteins to ensure minimal cross-reactivity. Cross-reactivity with other species, however, is possible.|
|Background||F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining.|
|Target||Rat IgG (F(ab')2)|
|Immunogen||Rat IgG F(ab')2 fragment|
|Applications||ELISA, IHC, WB|
Not tested in other applications.
|ELISA||1:10,000 - 1:100,000*
|Immunohistochemistry||1:500 - 1:5,000*
|Western blot||1:1,000 - 1:10,000*
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Storage Buffer||0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2 , 15 mg/mL Bovine Serum Albumin (BSA)|
|Storage Instruction||Keep as concentrated solution. For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.|
|Notes||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.|