|Application Note||Recommended Starting Dilutions:|
For WB: Use at a dilution of 1:1,000 (for ECL)
For IP: Use at 2 µL/250 µL of cell extract from 5 x 106 cells
For ICC/IF: Use at a dilution of 1:100-1:200
For IHC-P: Use at 1:1,000 (Heat-mediated antigen retrieval in Citrate Buffer (pH 6.3) is necessary)
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.