RhoA + RhoB + RhoC antibody [1A11-4G10]

Cat. No. GTX31128

GTX31128 WB Image

WB analysis of 25 μg of various whole cell lysates or 0.5 μg of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Dilution : 1:1000

1 / 8

GTX31128 IHC-P Image

IHC-P analysis of human hepatocarcinoma tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

2 / 8

GTX31128 ICC/IF Image

ICC/IF analysis of A431 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

3 / 8

GTX31128 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

4 / 8

GTX31128 IHC-P Image

IHC-P analysis of mouse breast tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

5 / 8

GTX31128 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

6 / 8

GTX31128 IP Image

IP analysis of HeLa cell lysate using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
IP reaction : 2μg antibody / 750μg lysate
Loading : 25 μg
Dilution : 1:1000

7 / 8

GTX31128 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

8 / 8

This RhoA + RhoB + RhoC antibody [1A11-4G10] (GTX31128) is a mouse monoclonal antibody validated for detection of RhoA + RhoB + RhoC by WB, ICC/IF, IHC-P, and IP. Reactivity has been shown in Human, Mouse, and Rat.

Host

Mouse
Clonality

Monoclonal
Clone Name

1A11-4G10
Isotype

IgG1
Applications

WB ICC/IF IHC-P IP
Reactivity

Human, Mouse, Rat