Immunocytochemistry/ Immunofluorescence : suggest to fix with methanol, not recommend to fix with PFANot tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||63 kDa (note)
|Positive Controls||293T , A431 , Jurkat , Raji , mouse brain , NCI-H929 , human Rhotekin-transfected 293T|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Product Description||Rabbit Polyclonal antibody to Rhotekin (rhotekin)|
|Background||This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]|
|Synonyms||RTKN antibody, rhotekin antibody|
|Immunogen||Recombinant protein encompassing a sequence within the N-terminus region of human Rhotekin. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rat (96%), Rhesus Monkey (88%), Bovine (84%)|