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1 .GTX129912 SA1 antibody

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SA1 antibody

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  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIP, WB
  • Species reactivityHuman
  • Publication  ( 1 )
Cat No: GTX129912
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-SA1 antibody used in Western Blot (WB). GTX129912
  • Anti-SA1 antibody used in Immunoprecipitation. GTX129912
  • Anti-SA1 antibody used in Western Blot (WB). GTX129912
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  • Bio-info
  • Protocol & MSDS
  • Application Information: SA1 antibody

Catalog Number GTX129912
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( 0 )
Publication ( 1 )
Product Name SA1 antibody
See all SA1 products
ApplicationsIP, WB
Application Note
Suggested dilutionReference
Immunoprecipitation1:100-1:500*  
Western Blot (WB)1:500-1:10000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size144 kDa (note)
Positive Controls293T , A431 , HeLa , HepG2
Form SuppliedLiquid
Concentration1.44 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: SA1 antibody

Full Namestromal antigen 1
BackgroundThis gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq]
SynonymsSCC3A Antibody , SA1 Antibody , stromal antigen 1 Antibody , STAG1 Antibody
Cellular LocalizationNucleus
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenCarrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SA1. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Mouse, Rat, Xenopus laevis
Predict Reactivity NoteMouse (100%), Rat (100%), Xenopus laevis (100%)
ConjugationUnconjugated

  • Storage Conditions: SA1 antibody

Storage Buffer1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Cell cycle > Cell division
  • Cell Biology > Cell cycle
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SA1 antibody validated data
  • Anti-SA1 antibody used in Western Blot (WB). GTX129912
    • GTX129912 WB Image
      Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody (GTX129912) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-SA1 antibody used in Immunoprecipitation. GTX129912
    • GTX129912 IP Image
      Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 μg of SA1 antibody (GTX129912).
      Western blot analysis was performed using SA1 antibody (GTX129912).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-SA1 antibody used in Western Blot (WB). GTX129912
    • GTX129912 WB Image
      Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody (GTX129912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-SA1 antibody used in Western Blot (WB). GTX129912
    • GTX129912 WB Image
      Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody (GTX129912) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-SA1 antibody used in Immunoprecipitation. GTX129912
    • GTX129912 IP Image
      Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 μg of SA1 antibody (GTX129912).
      Western blot analysis was performed using SA1 antibody (GTX129912).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-SA1 antibody used in Western Blot (WB). GTX129912
    • GTX129912 WB Image
      Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody (GTX129912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
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  • Reference for SA1 antibody
Application References Application Reactivity
Petronczki MP et al. Hardwired synthetic lethality within the cohesin complex in human cancer cells. bioRxiv (Epub ahead of print) (2017).
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensSTAG110274Q8WVM7, Q6P275604358NM_005862NP_005853144.45

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusStag120842Q6P5D1NM_009282NP_033308144.47
Rattus norvegicusStag1315958NM_001108179NP_001101649128.92
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-ProClin.pdf
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All products are for research use only—Not for use in diagnostic procedures.