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SBDS antibody [N1C3]

KO/KD
Anti-SBDS antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109168
Anti-SBDS antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109168
Anti-SBDS antibody [N1C3] used in Western Blot (WB). GTX109168
Anti-SBDS antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109168
Anti-SBDS antibody [N1C3] used in Western Blot (WB). GTX109168
Anti-SBDS antibody [N1C3] used in Western Blot (WB). GTX109168

Cat. No. GTX109168

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SBDS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

H1299 , HeLa , HepG2 , Mouse liver

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human SBDS Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951814

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SBDS ribosome maturation factor , CGI-97 , SDS , SWDS

Cellular Localization

Cytoplasm

Background

This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined. This gene has a closely linked pseudogene that is distally located. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SBDS antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109168

GTX109168 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using SBDS(GTX109168) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SBDS antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109168

GTX109168 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SBDS(GTX109168) antibody at 1:500 dilution.

Anti-SBDS antibody [N1C3] used in Western Blot (WB). GTX109168

GTX109168 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: HeLa
C: Hep G2 (GTX27900)
12% SDS PAGE
GTX109168 diluted at 1:1000

Anti-SBDS antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109168

GTX109168 IHC-P Image

SBDS antibody [N1C3] detects SBDS protein at cytosol on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SBDS antibody [N1C3] (GTX109168) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SBDS antibody [N1C3] used in Western Blot (WB). GTX109168

GTX109168 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX109168 diluted at 1:1000

Anti-SBDS antibody [N1C3] used in Western Blot (WB). GTX109168

GTX109168 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SBDS antibody [N1C3] (GTX109168) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB, ICC/IF, IP
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169