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SDHA antibody

KO/KD
Anti-SDHA antibody used in Western Blot (WB). GTX101689
Anti-SDHA antibody used in IHC (Paraffin sections) (IHC-P). GTX101689
Anti-SDHA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101689
Anti-SDHA antibody used in Western Blot (WB). GTX101689
Anti-SDHA antibody used in Western Blot (WB). GTX101689
Anti-SDHA antibody used in Western Blot (WB). GTX101689
Anti-SDHA antibody used in IHC (Paraffin sections) (IHC-P). GTX101689
Anti-SDHA antibody used in Western Blot (WB). GTX101689

Cat. No. GTX101689

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all SDHA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

Mouse brain , 293T

Predict Reactivity

Drosophila, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SDHA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241300

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

succinate dehydrogenase complex flavoprotein subunit A , CMD1GG , FP , PGL5 , SDH1 , SDH2 , SDHF

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SDHA antibody used in Western Blot (WB). GTX101689

GTX101689 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody (GTX101689) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody used in IHC (Paraffin sections) (IHC-P). GTX101689

GTX101689 IHC-P Image

SDHA antibody detects SDHA protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
SDHA antibody (GTX101689) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101689

GTX101689 ICC/IF Image

SDHA antibody detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SDHA protein stained by SDHA antibody (GTX101689) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-SDHA antibody used in Western Blot (WB). GTX101689

GTX101689 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX101689 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody used in Western Blot (WB). GTX101689

GTX101689 WB Image

SDHA antibody detects SDHA protein by Western blot analysis. Various whole cell extracts (30 ￡gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody (GTX101689) diluted by 1:500.

Anti-SDHA antibody used in Western Blot (WB). GTX101689

GTX101689 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody (GTX101689) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody used in IHC (Paraffin sections) (IHC-P). GTX101689

GTX101689 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using SDHA antibody (GTX101689) at 1:300 dilution.

Anti-SDHA antibody used in Western Blot (WB). GTX101689

GTX101689 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody (GTX101689) diluted at 1:500.

REFERENCE

Application Reference

Takuro Toda et al. Commun Biol 2022; 5 (1) : 453 Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria.
Application : WB
Imai Y et al. Commun Biol 2019; 2 : 424 Light-driven activation of mitochondrial proton-motive force improves motor behaviors in a Drosophila model of Parkinson's disease.
Sanchez I et al. Hum Mol Genet 2016; Ataxin-1 regulates the cerebellar bioenergetics proteome through the GSK3β-mTOR pathway which is altered in Spinocerebellar ataxia type 1 (SCA1).
Application : WB
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169